Coding

Part:BBa_K4743020:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-10-08)


crtE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 931


Design Notes

This sequence is optimized for saccharomyces cerevisiae

Source

Xanthophyllomyces dendrorhous

Accession number: AAY33921

References

1. Sandmann, G., & Misawa, N. (1992). New functional assignment of the carotenogenic genes crtB and crtE with constructs of these genes from Erwinia species. FEMS microbiology letters, 69(3), 253–257. https://doi.org/10.1016/0378-1097(92)90656-9

2. Math, S. K., Hearst, J. E., & Poulter, C. D. (1992). The crtE gene in Erwinia herbicola encodes geranylgeranyl diphosphate synthase. Proceedings of the National Academy of Sciences of the United States of America, 89(15), 6761–6764. https://doi.org/10.1073/pnas.89.15.6761